Nasturtium officinale Microshoot Culture Multiplied in PlantForm Bioreactor—Phytochemical Profiling and Biological Activity
Autor
Klimek-Szczykutowicz, Marta
Malinowska, Magdalena
Gałka, Aleksandra
Blažević, Ivica
Ðulović, Azra
Paprocka, Paulina
Wrzosek, Małgorzata
Szopa, Agnieszka
Opublikowane w
Molecules
Numeracja
30(4), 936
Data wydania
2025
Miejsce wydania
Basel, Switzerland
Wydawca
MDPI AG
Język
angielski
ISSN
1420-3049
DOI
https://doi.org/10.3390/molecules30040936
Słowa kluczowe
plant in vitro cultures, PlantForm temporary immersion system, antioxidant capacity; anti-elastase activity, anti-tyrosinase activity, anti-microbial activity, cosmetic formula
Abstrakt
Nasturtium officinale R. Br. (watercress) is an endangered species with valuable pharmaceutical, cosmetic, and nutritional properties. The purpose of this work was to evaluate the phytochemical profile and biological activity of extracts from microshoot cultures grown in PlantForm bioreactors and the parent plant material. After 20 days of cultivation, the cultures achieved the best results both in terms of key active ingredient content and biological activity. The glucosinolates (GSL) profile by the UHPLC-DAD-MS/MS method showed that the dominant compounds were glucobrassicin (493.00 mg/100 g DW, 10 days) and gluconasturtiin (268.04 mg/100 g DW, 20 days). The highest total polyphenol content (TPC) was obtained after a 20-day growth period (2690 mg GAE/100 g DW). Among polyphenols, the dominant compounds in the extracts from in vitro cultures were sinapinic acid (114.83 mg/100 g DW, 10 days) and ferulic acid (87.78 mg/100 g DW, 20 days). The highest antioxidant potential assessed by ABTS and DPPH assays was observed for ethanol extracts. The best results for inhibiting hyperpigmentation (18.12%) were obtained for ethanol extracts and anti-elastase activity (79.78%) for aqueous extract from N. officinale microshoot cultures. The extracts from microshoot cultures inhibited the growth of bacteria, including Cutibacterium acnes (MIC = 0.625 mg/mL). Antioxidant tests and the chelating capacity of iron ions Fe2+ of the face emulsion with N. officinale extracts showed higher results than the control.
