Physicochemical, structural and biological characterisation of poly(3-hydroxyoctanoate) supplemented with diclofenac acid conjugates - harnessing the potential in the construction of materials for skin regeneration processes
Physicochemical, structural and biological characterisation of poly(3-hydroxyoctanoate) supplemented with diclofenac acid conjugates - harnessing the potential in the construction of materials for skin regeneration processes
Physicochemical, structural and biological characterisation of poly(3-hydroxyoctanoate) supplemented with diclofenac acid conjugates - harnessing the potential in the construction of materials for skin regeneration processes
Autor
Haraźna, Katarzyna
Fricker, Annabelle T.
Konefał, Rafał
Medaj, Aneta
Zimowska, Małgorzata
Leszczyński, Bartosz
Wróbel, Andrzej
Bojarski, Andrzej J.
Roy, Ipsita
Guzik, Maciej
Opublikowane w
International Journal of Biological Macromolecules
Numeracja
Vol. 268, Part 1, 31476
Data wydania
2024
Wydawca
Elsevier B.V.
Język
angielski
ISSN
0141-8130
DOI
10.1016/j.ijbiomac.2024.131476
Słowa kluczowe
Polyhydroxyalkanoates, Poly(3-hydroxyoctanoate), Artificial skin substitute, bioresorbable, porous, layer of dressing material, Targeted delivery of diclofenac
Abstrakt
This study involved creating oligomeric conjugates of 3-hydroxy fatty acids and diclofenac, named Dic-oligo(3HAs). Advanced NMR techniques confirmed no free diclofenac in the mix. We tested diclofenac releaseunder conditions resembling healthy and chronic wound skin. These oligomers were used to make P(3HO)blends, forming patches for drug delivery. Their preparation used the solvent casting/porogen leaching (SCPL) method. The patches’ properties like porosity, roughness, and wettability were thoroughly analysed. Antimicrobial assays showed that Dic-oligo(3HAs) exhibited antimicrobial activity against reference (S. aureus, S. epidermis, S. faecalis) and clinical (Staphylococcus spp.) strains. Human keratinocytes (HaCaT) cell line tests, as per ISO 10993-5, showed no toxicity. A clear link between material roughness and HaCaT cell adhesion was found. Deep cell infiltration was verified using DAPI and phalloidin staining, observed under confocal microscopy. SEM also confirmed HaCaT cell growth on these scaffolds. The strong adhesion and proliferation of HaCaT cells on these materials indicate their potential as wound dressing layers. Additionally, the successful diclofenac release tests point to their applicability in treating both normal and chronic wounds.
